LCQ Advantage Max mass spectrometer - Wing4 (MS-MSn)

If you are new to the LCQ Max and you need access to it or you want to refresh your knowledge, please read the following guide.

How to operate the machine

Go to Operation procedure section.

Notes (FAQ)

  • Stay away from pollutants (except special cases).

How to gain access

Contact Jan.[1]

Booking system

Link to the booking system

Sample preparation

Please refer to the Sample preparation (MS, LC-MS).

Operation procedure

  • Inject some pure solvent to check for background contaminants.

  • Inject your sample.

  • Do advanced experiments.

  • Wash the instrument.

  • Inject pure solvent to check that instrument is clean.

Check Detailed procedure section for in-depth explanation.

Network storage

Please refer to the Network storage.

When something goes wrong

DON’T PANIC! ;) It happens to all of us someday. Contact Jan.[1]

Detailed procedure

  • Login with your science credentials.

  • Start windowsrdp (icon on desktop)

  • Check that the instrument is paused (icon #3 on image 1 is yellow and shows pause symbol)

  • Check that ionization mode is set to one which you want (icon #6 on image 1)

  • Open the Define Scan menu (icon #5 on image 1)

  • Edit the scan parameters (image 2 and 3)

  • Edit the source parameter (icon #1 on image 1, image 4)

  • Optional: edit injection control (trap parameters, icon #2 on image 1, image 5)

  • Use blank solvent and spray turn machine on (icon #3 on image 1, after click it should look like green play button) - ideal state: you have signal, but no specific intense ions.

  • Pause spraying (icon #3 on image 1, yellow pause), load your mixture.

  • Spray your mixture (icon #3 on image 1, green play), adjust parameters to get what you need.

  • Define recording (icon #4 on image 1).

  • Record your measurement.

  • Stop recording

  • Stop spraying

  • Flush the syringe.

  • Flush the inlet

  • Turn the spray on to check that everything is clean. (Important Step!)

  • Turn the spray off.

  • Remove solvent from syringe.

  • Change settings to default, check that everything is in stable state.

  • Copy your data to network share.

  • Close windowsrdp, logout.

../_images/xcalib_whole.png

Image 1: main window icons: 1) source parameters, 2) trap parameters, 3) on/pause switch, 4) recording, 5) scan parameters, 6) ionization mode switch.

../_images/scan_params.png

Image 2: Scan parameters, ms mode.

../_images/scan_params_msn.png

Image 3: Scan parameters, ms^n mode.

../_images/source_params.png

Image 4: ESI source parameters.

../_images/trap_params.png

Image 5: Trapping parameters.

Questions/Misc

If something is wrong in this guide feel free to contact Jan.[1]

TODO (planned improvements)

  • Make Prasopes compatible with the current setup.

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